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Thermo Scientific™ MspI (HpaII) (10 U/μL)

The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.

Brand: Thermo Scientific™ ER0542

1934.97 SEK valid until 2025-08-24
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Product Code. 10334330

2205.00 SEK / Pack of 5

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Description

Lambda DNA digested with MspI (HpaII), 1.4% agarose, 328 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C▵C G G...3'

3'...G G C▵C...5'

Conditions for 100% Activity:

1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer:

MspI is supplied in: 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

Dam: never overlaps —no effect
Dcm: never overlaps —no effect
CpG: completely overlaps —no effect
EcoKI: never overlaps —no effect
EcoBI: never overlaps —no effect

Compatible Ends:

Bsp119I, Bsu15I, Hin1I, Hin6I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Note:

MspI is an isoschizomer of HpaII. When the external C in the sequence CCGG is methylated, MspI and HpaII cannot cleave. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.

Specifications

Concentration	10 U/μL
Enzyme	MspI (HpaII)
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	10X Buffer Tango
Optimal Reaction Temperature	37°C
Quantity	5 x 3,000 units
Research Category	Traditional Cloning

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Documents

Product Information

Manuals

User Guide: MspI (HpaII), 10 U/uL, 3000U Version: Jan. 2015 File size: 70.6 kB

User Guide: MspI (HpaII), 10 U/uL, 5x3000U Version: Jan. 2015 File size: 70.6 kB

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2789461	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0542
Lot Number2829737	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0542
Lot Number2781132	Certificate TypeCertificate of Analysis	Date09/08/2023	Catalog NumberER0542
Lot Number2744434	Certificate TypeCertificate of Analysis	Date16/06/2023	Catalog NumberER0542
Lot Number2734272	Certificate TypeCertificate of Analysis	Date01/06/2023	Catalog NumberER0542

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Thermo Scientific™ MspI (HpaII) (10 U/μL)

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