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Thermo Scientific™ X-Gal Solution, ready-to-use

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Brand: Thermo Scientific™ R0941

Product Code. 10508750

1370.00 SEK / 10mL

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Description

Formula: C₁₄H₁₅BrClNO₆ Molecular weight: 408.6

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Recommended for:

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.

Note:

Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

Specifications

Detection Method	Colorimetric
Quantity	10 mL
Substrate Type	Beta-Gal Substrate, X-Gal

Product Type	X-Gal Solution
Substrate Properties	Chemical Substrate

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

X-Gal Solution, ready-to-use

Signal Word

Warning

Hazard Category

Flammable liquid Category 3
Skin sensitiser Category 1

Hazard Statement

H226-Flammable liquid and vapour.
H317-May cause an allergic skin reaction.

Precautionary Statement

P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233-Keep container tightly closed.
P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P272-Contaminated work clothing should not be allowed out of the workplace.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P370+P378-In case of fire: Use dry sand, dry chemical, or alcohol-resistant foam to extinguish.
P403+P235-Store in a well-ventilated place. Keep cool.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : ethanol, hydroquinone, DMSO

Documents

Product Information

Manuals

User Guide: X-Gal Solution - ready-to-use Version: Jan. 2015 File size: 50.6 kB

User Guide: Detect beta-galactosidase Report Gene Transfected Eukaryotic Cells Version: Jan. 2015 File size: 74.3 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2796035	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberR0941
Lot Number2822302	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberR0941
Lot Number2855266	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberR0941
Lot Number2873608	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberR0941
Lot Number2906597	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberR0941

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