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Thermo Scientific™ T3 RNA Polymerase, HC (≥100 U/μL)

Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Brand:  Thermo Scientific™ EP0103

1229.30 SEK valid until 2024-09-17
Use promo code "21690" to get your promotional price.



Additional Details : Weight : 0.01000kg

Product Code. 10680101

  • 1294.00 SEK / 10000 units
Estimated Shipment: 06-08-2024
to see stock.

Description

Description

Thermo Scientific™ Fermentas Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Specifications

Specifications

T3 RNA Polymerase
10,000 U
200U/µL
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For Research Use Only. Not for use in diagnostic procedures.