Läs mer
Thermo Scientific™ T3 RNA Polymerase
Handla allt Thermo Scientific ProdukterBeskrivning
Thermo Scientific™ Fermentas Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.
- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Recommended for:
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Note:
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
Specifikationer
Specifikationer
| Koncentration | 20U/µL |
| Beskrivning | T3 RNA Polymerase |
| För användning med (applikation) | Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing. |
| Inkluderar | 1mL of 5X Transcription Buffer |
| Kvantitet | 10,000 U |
Din input är viktig för oss. Fyll i det här formuläret för att ge feedback relaterad till innehållet på denna produkt.