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Fisher BioReagents™ Taq DNA Polymerase

Allows up to 100 cycles of amplification without loss of activity

402.00 SEK - 43405.00 SEK

Specifications

Concentration 5U/μL
Format Liquid
For Use With (Application) PCR
Product Name Taq DNA Polymerase
Storage Buffer Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
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Products 14
Product Code Brand Formulation Quantity Price Quantity & Availability  
Product Code Brand Formulation Quantity Price Quantity & Availability  
10717185
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Fisher BioReagents™
FB6000-20
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 25 × 100 Units
9130.00 SEK
2500 units
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Estimated Shipment: 17-10-2022
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10104974
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Fisher BioReagents™
FB6000-75
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 2500 Units
43405.00 SEK
Pack of 5
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Estimated Shipment: 17-10-2022
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10458923
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Fisher BioReagents™
FB6000-30
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 500 Units This item is currently unavailable or has been discontinued.
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10091263
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Fisher BioReagents™
FB6000-15
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 100 Units This item is currently unavailable or has been discontinued.
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10458733
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Fisher BioReagents™
FB6000-25
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 500 Units This item is currently unavailable or has been discontinued.
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10698393
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Fisher BioReagents™
FB6000-35
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 2500 Units This item is currently unavailable or has been discontinued.
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10144924
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Fisher BioReagents™
FB6000-40
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 5 × 2500 Units This item is currently unavailable or has been discontinued.
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10449693
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Fisher BioReagents™
FB6000-45
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 100 Units This item is currently unavailable or has been discontinued.
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10346363
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Fisher BioReagents™
FB6000-50
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 100 Units This item is currently unavailable or has been discontinued.
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10216744
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Fisher BioReagents™
FB6000-70
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 2500 Units This item is currently unavailable or has been discontinued.
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10356363
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Fisher BioReagents™
FB6000-60
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 500 Units This item is currently unavailable or has been discontinued.
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10206744
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Fisher BioReagents™
FB6000-55
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 25 × 100 Units This item is currently unavailable or has been discontinued.
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10448733
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Fisher BioReagents™
FB6000-10
10X assay buffer A : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 15mM solution in a separate vial 100 Units This item is currently unavailable or has been discontinued.
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10071553
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Fisher BioReagents™
FB6000-65
10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl2 25mM solution in a separate vial 5 × 500 Units This item is currently unavailable or has been discontinued.
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Description

Description

  • Licensed for use in PCR
  • Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
  • Has a 5«→3« exonuclease activity
  • Available with two different reaction buffers
  • Buffer A contains an optimized final concentration of 15mM MgCl2 for most PCR amplifications
  • Buffer B does not contain MgCl2; MgCl2 is provided in a separate vial as a 25mM solution
  • Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
  • Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl2) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl2 solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

Specifications

Specifications

5U/μL
PCR
Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%
Liquid
Taq DNA Polymerase
-20°C
SDS
Product Certifications