missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Brand: Invitrogen™ 65-0863-14
1275.01 SEK valid until 2024-04-30
Use promo code "21741" to get your promotional price.
Additional Details : Weight : 1.00000kg
Description
- Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
- Thus, using these dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
- Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™)
- For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
- If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
- After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
- Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
- It should be protected from light and moisture
- Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
- Allow vial to equilibrate to room temperature before opening
Specifications
| Blue | |
| Viability Assay | |
| Dye | |
| eBioscience™ |
| Fluorescent | |
| Flow Cytometer | |
| 405/450 | |
| 100 Tests |
For Research Use Only. Not for use in diagnostic procedures.