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FLT3 [ITD] Recombinant Human Protein

FLT3 [ITD] Recombinant Human Protein

Brand:  Invitrogen PV6191

UNSPSC : 12352202

Additional Details : Weight : 0.01000kg

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Product Code. 15458863

  • 10180.00 SEK / 100µg


Estimated Shipment date
from Supplier 09-10-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

Human FLT3, C-terminal fragment, amino acids R571-S993 (as in NCBI/Protein entry NP_004110.2), internal tandem duplication (oncological mutation: ITD) of aa 591-600 N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use

    Related Product:
    LanthaScreen™ Eu Kinase Binding Assay
  • Specifications

    Specifications

    NP_004110.2
    Pharma and biopharma, protein assays and analysis, target and lead identification and validation
    FLT3 [ITD] Recombinant Human Protein
    –68 to –85°C
    1 Each
    FLT3 [ITD] Recombinant Human Protein
    Kinase assay
    FLT3 [ITD] Recombinant Human Protein
    Human
    0.095mg/mL
    78.6kDa
    100μg
    For research use only. Not for use in diagnostic procedures
    CD135, EC 2.7.10.1, FLK2, STK1
    FLT3 ITD was assayed for activity at 1.0μg/mL in 60mM HEPES (pH 7.5), 3mM MgCl2, 3mM MnCl2, 3μM Na3VO4, 1.2mM DTT, 50μg/mL PEG20,000, ATP (titrated) using 10μg/mL poly(Ala, Glu, Lys, Tyr)6:2:5:1 substrate
    Amino acids 571-993
    15nmoles of phosphate transferred to poly(Ala, Glu, Lys, Tyr)6:2:5:1 substrate per minute per mg of total protein at saturating ATP
    GST Tag, His Tag