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Invitrogen™ FokI Polyclonal Antibody
Rabbit Polyclonal Antibody
Brand: Invitrogen™ PA5119997
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Beskrivning
Positive Control: Purified restriction enzyme FokI.
The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
Specifikationer
| FokI | |
| Polyclonal | |
| Unconjugated | |
| Fok1 | |
| Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR. | |
| 100 μL | |
| Primary | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid |
| Western Blot | |
| 1 mg/mL | |
| PBS with 0.2% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4 | |
| Rabbit | |
| Affinity chromatography | |
| RUO | |
| Bacteria | |
| Antibody | |
| IgG |
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