FOXP3, clone: 236A/E7, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-4777-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Tested: This 236A/E7 antibody has been tested by immunoblotting and immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval. For western blotting of reduced cell lysates (which include the nuclear fraction) the antibody can be used at 1-10 ug/mL and for IHC can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID|
|HumanþNon-human PrimateþRhesus Monkey|
|Immunohistochemistry (Frozen)þImmunohistochemistry (Paraffin)þWestern Blot|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.