FOXP3 Rat anti-Bovine, Canine, Feline, Mouse, Porcine, Rat, Biotin, Clone: FJK-16s, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 13-5773-80
Additional Details : Weight : 0.25000kg
DescriptionApplications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using Foxp3/Transcription Factor buffer set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The FJK-16s antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. The FJK-16s antibody has been tested by immunohistochemistry of frozen mouse spleen and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered. FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID|
|Bovine, Canine, Feline, Mouse, Porcine, Rat|
|Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot|
|4° C, store in dark, DO NOT FREEZE!|
|100037405, 20371, 317382, 444998, 491876, 506053|
For Research Use Only.