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FOXP3, clone: FJK-16s, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5773-80

Additional Details : Weight : 0.01000kg

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Product Code. 15237457

  • 1212.00 SEK / 25µg


EU Stock 4
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Description

Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Applications Reported: This FJK-16s antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. For additional information, please see Foxp3 FAQs.Applications Tested: This FJK-16s antibody has been tested by immunoblotting (WB) (1-5 μ/mL) of mouse splenocytes. The FJK-16s antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Foxp3
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q99JB6
AIID, DIETER, Forkhead box P3, foxp3, IPEX, JM2, PIDX, SCURFIN, XPID
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Canine, Feline, Murine, Porcine, Rat
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Western Blot
FJK-16s
Unconjugated
Foxp3
Liquid
FOXP3
IgG2a, kappa, kappa
25μg
4° C
Primary
100037405, 20371, 317382, 444998, 491876, 506053
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