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FR4 Monoclonal Antibody (eBio12A5), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-5445-82
Additional Details : Weight : 0.25000kg
Description
Description: The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR delta and FBP3 (folate binding protein3). FR4 is a heavily glycosylated, 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural Treg have high levels of FR4 and together with CD25 can be used to distinguish natural Treg, effector T cells, memory-like T cells and naive T cells. FR4 high CD25 high cells are natural Treg with high levels of Foxp3. FR4 high CD25 low cells are identified as central memory T cells, which upon stimulation, can proliferate and produce large amounts of IL-2. In contrast, FR4 low CD25 high cells secrete proinflammatory cytokines such as IFN gamma and IL-17 and have been characterized as effector memory T cells. Based on co-staining studies, the epitopes recognized by eBioTH6 and eBio12A5 are different, thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5. Applications Reported: This eBio12A5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio12A5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate. Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naive T cells. In vivo studies with eBioTH6 show a decrease in the number of CD4+CD25+ cells and an enhanced tumor response leading to the elimination of advanced stages of tumors.Specifications
FR4 | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Q9EQF4 | |
Izumo1r | |
Affinity chromatography | |
RUO | |
64931 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBio12A5 | |
FITC | |
Izumo1r | |
0910001L11Rik; C86255; Folate receptor 4; folate receptor 4 (delta); folate receptor 4 (delta) homolog; folate receptor 4, delta (putative); folate receptor delta; Folate-binding protein 3; Folbp3; Folr4; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1 receptor, JUNO; Izumo1r; Juno; probable folate receptor delta; sperm-egg fusion protein Juno | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.