Galectin 9 Monoclonal Antibody (9M1-3), Functional Grade, eBioscience™, Invitrogen™

Description

Description: This 9M1-3 monoclonal antibody reacts with human Galectin-9. Galectin-9 is one of fifteen members of the galectin family of glycan-binding proteins. It is expressed on activated T helper cells and Foxp3+ T regulatory cells and plays a critical role in the regulation of immune function. Interaction of Galectin-9 with its ligand, TIM3, induces apoptosis in Th1 and Th17, inhibits Th17 polarization and IFN gamma expression, and drives Treg expansion. Galectin-9 is also thought to contribute to the survival and expansion of some tumors by inhibiting the anti-tumor response and promoting immune evasion. Along with Galectin-1 and Galectin-3, high levels of Galectin-9 expression are associated with poor cancer prognosis, making these potential therapeutic targets. Applications Reported: This 9M1-3 antibody has been reported for use in flow cytometric analysis and functional assays. (Fluorochrome-conjugated 9M1-3 is recommended for use in intracellular flow cytometry.). Applications Tested: This 9M1-3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.