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TCR gamma/delta, PerCP-eFluor™ 710, clone: B1.1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9959-41

Additional Details : Weight : 0.23750kg

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Product Code. 15509476

  • 1038.00 SEK / 25 tests


EU Stock 4
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Description

Description

Description: The B1.1 monoclonal antibody reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection. This monoclonal can be used as a phenotypic marker for gamma delta TCR-expressing T cells.Applications Reported: This B1.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (2.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

gamma delta TCR
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Conjugated
TRD
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6964
Flow Cytometry
B1.1
PerCP-eFluor 710
TRD
T-cell receptor gamma, t-cell receptor delta, TCRd, TCRg, TCR delta gamma, TCR-CD3 complex
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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