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Invitrogen™ Gateway™ pENTR™ 3C Dual Selection Vector

Gateway™ pENTR™ 3C Dual Selection Vector

Brand:  Invitrogen™ A10464

Product Code. 10338413

  • 602.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:

  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    • o negative selection and
    • o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Dual SelectionExpression Vector
Chloramphenicol (CmR), Kanamycin (KanR)
Untagged
10 μg
pENTR™
10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.
Store at -20°C
No Cleavage Site
Gateway
pENTR
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Product Information

Manuals

Gateway pENTR Dual Selection Vectors Version: Version A, 6 June 2008 File size: 456.6 kB
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For Research Use Only. Not for use in diagnostic procedures.