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GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Brand:  GE Healthcare 27-0846-01

Additional Details : Weight : 0.50000kg

Product Code. 10218854

  • 1221.00 SEK / 500 units


Sweden Stock
(immediate availability): 8
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Description

Description

  • Supplied as lyophilized powder
  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

Specifications

Specifications

Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3
Thrombin Protease Fusion Protein
500U
Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours
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