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Cytiva (Formerly GE Healthcare Life Sciences) Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Brand:  Cytiva (Formerly GE Healthcare Life Sciences) 27-0846-01

Additional Details : Weight : 0.50000kg

Product Code. 10218854

  • 1221.00 SEK / 500 units

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  • Supplied as lyophilized powder
  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow



Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3
Thrombin Protease Fusion Protein
Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours
Safety and Handling

Safety and Handling

Signal Word
  • Danger

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : thrombin