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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
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Kvantitet:
10 reactions
Förpackningsstorlek:
10 reaktioner
Beskrivning
Innehåller
- CRISPR CD4 Nuclease Vector, linearized
- 10X Oligonucleotide Annealing Buffer
- DNase/RNase-free Water
- 5X Ligation Buffer
- T4 DNA Ligase
- U6 Forward Sequencing Primer
- Control Double-stranded Oligonucleotide
- OneShot™ TOP10 Chemically Competent Cells
Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. Competent cells included for added convenience and highest cloning efficiencies.
GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).
The GeneArt™ CRISPR Nuclease Vector system with competent cells offers
GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).
The GeneArt™ CRISPR Nuclease Vector system with competent cells offers
- Easy-to-design genome engineering system
- Affordable, ready-to-use cloning vectors
- Enrichment for hard to transfect or difficult to modify cell lines
Certifikat
CoA
Uppfyllande
CoA
Rekommenderad förvaring
Store in freezer (-5 to -30°C)
Specifikationer
Specifikationer
| Kloningsmetod | Restriction Enzyme/MCS |
| Innehåll och lagring | • CRISPR CD4 Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control, double stranded oligonucleotide Store the above at -5 to -30°C. • OneShot™ TOP10 Chemically Competent Cells Store at -80°C. |
| Detektionsmetod | Primer-probe |
| Form | Liquid |
| Formatera | Kit |
| Reaktionshastighet | Fast |
| Teknik | CRISPR-Cas9 |
| Produktlinje | GeneArt™ |
| Produkttyp | Crispr Nuclease Vector With CD4 Enrichment Kit |
| Promotor | U6, CMV |
| Visa mer |
For Research Use Only. Not for use in diagnostic procedures.
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