Invitrogen™ GeneArt™ Platinum™ Cas9 Nuclease (3 µg/µL)

Description

Maximum efficient, the Cas9 RNP complex minimizes off-target cleavage compared to vector-based systems.

GeneArt Platinum Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The Cas9 RNP complex can act immediately once it enters the cell since there are no requirements for transcription or translation as with vector- or mRNA-based systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cell minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). Incorporation of a nuclear localization signal (NLS) aids delivery to the nucleus, thus increasing the rate of genomic DNA cleavage.

GeneArt Platinum Cas9 Nuclease features include:

• Streamline cell engineering by eliminating transcription and translation in the cell
• Eliminate time consuming cloning steps
• Minimize off-target cleavage due to rapid clearance of the protein complex from the cell

Certifications

CoA

Compliance

CoA

Recommended Storage

Store at -5 to -30°C