missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

The GeneBLAzer™ ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

Brand:  Thermo Scientific™ K1577

Product Code. 10465575

  • 8180.00 SEK / Each
Estimated Shipment: 29-10-2024
to see stock.
Explore more special offers
Add to basket

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The GeneBLAzer™ ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession No. NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

NM_000680
Division-Arrested Cells
GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, GeneBLAzer™ System, Liquid Nitrogen Storage
Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ ADRA1A CHO-K1DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ ADRA1A CHO-K1Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
GeneBLAzer™
BLA (Beta-Lactamase)
ADRA1A
CHO-K1
GPCR
Cell-based beta lactamase reporter gene
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ ADRA1A CHO-K1DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ ADRA1A CHO-K1Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ ADRA1A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Fluorescence
ADRA1A
Pool of Stable Cells
1 Plate
CHO-K1
Division-Arrested Cells Assay Kit
GeneBLAzer™
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.