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Gibco™ DMEM/F-12, HEPES, no phenol red

Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: L-glutamine, HEPES
Without: Phenol Red
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
- Environmental benefits include:
- Renewable Materials
- Recycled Content
- Source Reduction
- Recyclable

Specifications
Specifications
Cell Line | MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts |
Classification | Animal Origin-free |
Concentration | 1X |
Form | Liquid |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Serum Level | Standard Serum Supplementation |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose, Glutamine, HEPES |
Without Additives | No Phenol Red, No Sodium Pyruvate |
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Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2660120 | Certificate of Analysis | 29/01/2025 | 11039021 |
2661461 | Certificate of Analysis | 29/01/2025 | 11039021 |
2731402 | Certificate of Analysis | 28/01/2025 | 11039021 |
2828793 | Certificate of Analysis | 28/01/2025 | 11039021 |
2715725 | Certificate of Analysis | 27/01/2025 | 11039021 |
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For Research Use Only. Not for use in diagnostic procedures.
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