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Gibco™ DMEM, low glucose, pyruvate, HEPES

Description
Requires
Supplementation
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: Low Glucose, L-glutamine, Sodium Pyruvate, HEPES, Phenol Red
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
- Environmental benefits include:
- Renewable Materials
- Recycled Content
- Recyclable
- Source Reduction

Specifications
Specifications
Cell Line | HeLa, 293, Cos-7, and PC-12 |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
Classification | Animal Origin-free |
Concentration | 1 X |
Form | Liquid |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Serum Level | Standard Serum Supplementation |
Sterility | Sterile-filtered |
With Additives | Low Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2451115 | Certificate of Analysis | 29/01/2025 | 12320032 |
2323513 | Certificate of Analysis | 29/01/2025 | 12320032 |
2766558 | Certificate of Analysis | 29/01/2025 | 12320032 |
2887923 | Certificate of Analysis | 25/01/2025 | 12320032 |
2998654 | Certificate of Analysis | 25/01/2025 | 12320032 |
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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