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Gibco™ MEM α, nucleosides, no ascorbic acid Product Code.: 10712124

Gibco™ MEM α, nucleosides, no ascorbic acid
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Product Code. 10712124
500 mL, 500mL
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Quantity:
500 mL
Unit Size:
500mL
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10712124

Brand: Gibco™ A1049001

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Requires

Supplementation

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Deoxyribonucleosides, Ribonucleosides, L-glutamine, Phenol Red

Without: Ascorbic acid

The complete formulation is available.

Using MEM α

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Greener Choice Claims
  • Environmental benefits include:
    • Recycled Content
    • Renewable Materials
    • Recyclable
    • Source Reduction
TRUSTED_SUSTAINABILITY

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM α (Minimum Essential Medium α)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides
Without Additives No Ascorbic Acid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2323447 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeA1049001
Lot Number2559744 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeA1049001
Lot Number2445677 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeA1049001
Lot Number2451171 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeA1049001
Lot Number2766705 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeA1049001

For Research Use Only. Not for use in diagnostic procedures.

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