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Gibco™ MEM, suspension, no calcium, no glutamine Product Code.: 10013082
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Gibco™ MEM, suspension, no calcium, no glutamine
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Product Code. 10013082
500 mL, 500mL
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Quantity:
500 mL
Unit Size:
500mL
This item is not returnable. View return policy

Product Code. 10013082

Brand: Gibco™ 11380037

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This item is not returnable. View return policy

Description

Requires

Supplementation

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Earle's salts, Phenol Red

Without: L-glutamine, HEPES, Calcium

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle's salts for use in a CO2 incubator. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Greener Choice Claims
  • Environmental benefits include:
    • Renewable Materials
    • Recycled Content
    • Recyclable
    • Source Reduction
TRUSTED_SUSTAINABILITY

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM (Minimum Essential Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives Low Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without Additives No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.

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