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GL7, clone: GL-7 (GL7), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5902-81

Additional Details : Weight : 0.01000kg

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Product Code. 15277587

  • 586.00 SEK / 50µg


Estimated Shipment date
from Supplier 22-11-2018
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Description

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

GL7
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Ly-77, Ly77, B cell activation marker, GL7 antigen
IgM
50μg
4° C
Primary
Human, Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
GL-7 (GL7)
Unconjugated
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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