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GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

770.00 SEK - 2915.00 SEK

Specifications

Antigen GL7
Clone GL-7 (GL7)
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
Classification Monoclonal
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15277587
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Affymetrix eBioscience
14-5902-81
50 μg
770.00 SEK
50µg
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Estimated Shipment: 08-05-2024
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15287587
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Affymetrix eBioscience
14-5902-82
100 μg
1120.00 SEK
100µg
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Estimated Shipment: 10-04-2024
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15297587
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Affymetrix eBioscience
14-5902-85
500 μg
2915.00 SEK
500µg
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Estimated Shipment: 10-04-2024
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Description

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
Specifications

Specifications

GL7
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
IgM
Affinity chromatography
Antibody
GL-7 (GL7)
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
Unconjugated
Rat
Human, Mouse
GL7 antigen; Ly77; Ly-77
Primary
4° C
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.