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GFAP Monoclonal Antibody (GA5), eFluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-9892-80
Additional Details : Weight : 0.09500kg
Description
Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predomit form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This GA5 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 660 is a replacement for Alexa Fluor™ 647.
GFAP Monoclonal antibody specifically detects GFAP in Chicken, Human, Mouse, Porcine, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).Specifications
GFAP | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P03995, P14136, P47819 | |
GFAP | |
Affinity chromatography | |
RUO | |
100352532, 14580, 24387, 2670, 396562, 419969 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) | |
GA5 | |
eFluor 660 | |
GFAP | |
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen | |
Mouse | |
25 μg | |
Primary | |
Chicken, Human, Mouse, Porcine, Rabbit, Rat | |
Antibody | |
IgG1 |
For Research Use Only.