GM-CSF Rat anti-Mouse, PE, Clone: MP1-22E9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-7331-41
Additional Details : Weight : 0.23750kg
DescriptionDescription: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.Applications Reported: MP1-22E9 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MP1-22E9 antibody is tested by intracellular staining and flow cytometric analysis of cultured mouse splencoytes. This product has been pre-titrated and tested bu intracellular staining and flow cytometric analysis of cultured mouse splencoytes. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Granulocyte Macrophage Colony Stimulating Factor, Colony-stimulating factor, CSF, Sargramostim, Molgramostin|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.