Goat anti-Mouse IgG (H+L) Secondary Antibody, PE, Invitrogen™

Description

PA1-84395 has been absorbed to minimize cross-reactivity with rat IgG. Reconstitute with 1.0 mL of distilled water. After reconstitution, store product undiluted at 4oC in the dark. Once reconstituted, this product has a shelf life of approx. 18 months. For FACS analysis, use 50 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. RPE conjugated Goat anti Mouse IfF antibody recognizes both the heavy and light chains of mouse IgG.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.