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Granzyme A, eFluor 450, clone: GzA-3G8.5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5831-80

Additional Details : Weight : 0.09500kg

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Product Code. 15519776

  • 1022.00 SEK / 25µg


Estimated Shipment date
from Supplier 24-08-2018
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Description

Description

Description: This Gza-3G8.5 monoclonal antibody reacts with mouse Granzyme A. Granzyme A is the most abundantly expressed of the ten granzyme serine proteases that have been identified in mice. Granzymes are proteins released from the granules of NK cells and cytotoxic T lymphocytes that induce death in target cells by cleavage of intracellular substrates and play a critical role in immune defense against viruses, tumors, and intracellular bacteria. Granzyme A activates caspase-independent cell death pathways morphologically similar to apoptosis and characterized by mitochondrial and DNA damage. It may also play a role in inflammation, as the precursor form of IL-1 beta (pro-IL-1 beta) is among its target substrates. Granzyme A shares overlapping substrate specificity with the closely-related Granzyme K, which is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs.Applications Reported: This GzA-3G8.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This GzA-3G8.5 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Granzyme A
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11032
CTLA3, HFSP
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFlow Cytometry
GzA-3G8.5
eFluor 450
Gzma
Conjugated
Gzma
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14938
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