Invitrogen™ Granzyme B Monoclonal Antibody (GB11), PE, eBioscience™

Mouse Monoclonal Antibody

Description: The GB11 antibody reacts with human Granzyme B (GrB). GrB is one of the family of serine proteases, known as granzymes, that is located in the granules of cytotoxic T cells and NK cells. Granzyme B has been described also as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis. Applications Reported: The GB11 antibody has been reported useful for intracellular staining followed by flow cytometric analysis. Applications Tested: This GB11 antibody has been pre-titrated and tested on by intracellular staining and flow cytometric analysis of both cultured normal human peripheral blood cells and mouse splenocytes. This can be used at test size: 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; L...