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Granzyme B Rat anti-Mouse, Clone: 16G6, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-8822-80

Additional Details : Weight : 0.01000kg

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Product Code. 15248097

  • 792.00 SEK / 25µg


Estimated Shipment date
from Supplier 31-07-2019
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Description

Description

Applications Tested: The unlabelled 16G6 antibody has been tested as the capture antibody in a sandwich ELISA for quantitation of mouse granzyme B protein levels, in combination with the biotinylated eBioLUEE (13-8821) antibody for detection and recombit mouse granzyme B as the standard. A suitable range of concentrations of this antibody for ELISA detection is 1-4 µg/mL. The 16G6 antibody has been tested for Western blotting; a recommended starting concentration is 1 µg/mL. The 16G6 antibody has been used for formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Specifications

Specifications

Granzyme B
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04187
GrB
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
ELISA, Immunohistochemistry (Paraffin), Western Blot
16G6
Unconjugated
Gzmb
Liquid
Gzmb
IgG2b, kappa
25 μg
4° C
Primary
14939
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