TCR H-Y (male antigen) Mouse anti-Mouse, FITC, Clone: T3.70, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-9930-81
Additional Details : Weight : 0.25000kg
Description: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation. Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis. Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.
|TCR H-Y (male antigen)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Male antigen HY; TCR HY|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.