HELIOS, eFluor 450, clone: 22F6, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 48-9883-42
Additional Details : Weight : 0.10000kg
DescriptionDescription: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.22F6 has been reported to cross react with rat Helios.Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using Foxp3 Fixation and Permeabilization Buffers (cat. 00-5521). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|HELIOS, ZNF1A2, ZNFN1A2, ikaros family zinc finger protein 2, zinc finger DNA binding protein Helios, zinc finger protein Helios, zinc finger protein, subfamily 1A, 2 (Helios)|
|Canine, Human, Murine|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|22779, 22807, 488510|
For Research Use Only.