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HELIOS Armenian Hamster anti-Canine, Human, Mouse, PE-eFluor® 610, Clone: 22F6, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 61-9883-41

Additional Details : Weight : 0.23750kg

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Product Code. 15500587

  • 1486.00 SEK / 25 tests


Estimated Shipment date
from Supplier 02-07-2019
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Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predomitly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Specifications

Specifications

Helios
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9UKS7, P81183
HELIOS, ZNF1A2, ZNFN1A2, ikaros family zinc finger protein 2, zinc finger DNA binding protein Helios, zinc finger protein Helios, zinc finger protein, subfamily 1A, 2 (Helios)
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Human, Mouse
Flow Cytometry
22F6
PE-eFluor® 610
IKZF2
Liquid
IKZF2
IgG
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
22779, 22807, 488510
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