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HLA-ABC Monoclonal Antibody (W6/32), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

2890.00 SEK

Specifications

Antigen HLA-ABC
Clone W6/32
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 1
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15558706
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Affymetrix eBioscience
25-9983-42
100 Tests
2890.00 SEK
100 tests
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Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

HLA-ABC Monoclonal antibody specifically detects HLA-ABC in Human samples. It is validated for Flow Cytometry.
Specifications

Specifications

HLA-ABC
5 μL/Test
Monoclonal
Liquid
RUO
IgG2a κ
Affinity chromatography
Antibody
W6/32
Flow Cytometry
PE-Cyanine7
Mouse
Human
Primary
4° C, store in dark, DO NOT FREEZE!
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