eBioscience™ SAFE™ Human TNF alpha, Recombinant Protein
Human Recombinant Protein
Brand: Affymetrix eBioscience 68-8786-63
Additional Details : Weight : 0.09500kg
DescriptionThis SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.
Human TNF alpha is a memeber of the TNF ligand superfamily produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 24 kDa form of TNF alpha is expressed as a membrane bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Human and mouse TNF alpha conservation is about 79%, Human protein is functional on mouse, although with slightly reduced activity.
|The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile PBS to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots|
|Plant expressed 158 amino acids (Val77-Leu233) with 16 amino acid Histidine tag. Accession P01375|
|Tumor Necrosis Factor alpha|
|Sterile lyophilized (PBS buffer pH 7.2)|
|Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay|
|The ED50 of this protein, as measured by cytolystic activity of actinomycin-D sensitized L929 cells, is less than or equal to 0.4ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 106 Units/mg.|