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Invitrogen™ Cytochrome C Human ELISA Kit
For the quantitative detection of human Cytochrome c.
Brand: Invitrogen™ BMS263TEN
Additional Details : Weight : 0.23750kg
Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cytochrome c
Biotin-Conjugate anti-human Cytochrome c monoclonal antibody
Streptavidin-HRP
Human Cytochrome c Standard lyophilized, 10ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Green-Dye
Red-Dye
Adhesive Films
Description
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.Specifications
| P99999 | |
| Biotin | |
| 54205 | |
| ELISA Kit | |
| 0.05 ng/mL | |
| Cell Lysate,5 μL | |
| 1 hr. 20 min. | |
| Human | |
| CYC, somatic Cytochrome C, HCS | |
| 6% | |
| Human | |
| HRP |
| 0.08-5.0 ng/mL | |
| ELISA | |
| Pre-coated 96 well plates, Controls, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution | |
| RUO | |
| 100 μL (prediluted) | |
| 2°C to 8°C | |
| 3 hr. 10 min. | |
| CYC,HCS,THC4 | |
| 4% | |
| 10 x 96 Tests | |
| Cell Lysates | |
| Colorimetric Microplate Reader |
For Research Use Only