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Invitrogen™ Cytochrome C Human ELISA Kit

For the quantitative detection of human Cytochrome c.

Brand:  Invitrogen™ BMS263TEN

27634.71 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.23750kg

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Product Code. 15561847

  • 41450.00 SEK / Pack of 10
Estimated Shipment: 11-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cytochrome c
Biotin-Conjugate anti-human Cytochrome c monoclonal antibody
Streptavidin-HRP
Human Cytochrome c Standard lyophilized, 10ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human Cytochrome c ELISA quantitates Hu cytochrome c in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu cytochrome c. Principle of the method The Human cytochrome c solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
Specifications

Specifications

P99999
0.05 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CYC,HCS,THC4
6%
HRP
RUO
2°C to 8°C
Human
3 hr. 10 min.
0.08-5.0 ng/mL
Biotin
Cell Lysates
ELISA
54205
4%
Pre-coated 96 well plates, Standard, Sample Diluent, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Lysis Buffer, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution
10 x 96 Tests
Cell Lysate,5 μL
CYC, somatic Cytochrome C, HCS
1 hr. 20 min.
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