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Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™, Invitrogen™

Polyclonal Antibody

Brand:  Affymetrix eBioscience 14-9161-71

600.03 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.01000kg

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Product Code. 15218157

  • 900.00 SEK / 25 tests
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Alert:

Contains Azide


Description

Description

Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs. Applications Reported: Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody. Applications Tested: Purified human Fc gammaR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μL per test.

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
Specifications

Specifications

Fc Receptor Binding Inhibitor
Polyclonal
Unconjugated
Fc blocking; FcR BI; FcR Inhibitor
Affinity chromatography
RUO
Human
Antibody
Flow Cytometry
1 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Human
25 Tests
Primary
4° C
Liquid
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