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Fc Receptor Binding Inhibitor anti-Human, Polyclonal, eBioscience™

Polyclonal Antibody

Brand:  Affymetrix eBioscience 14-9161-71

Additional Details : Weight : 0.01000kg

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Product Code. 15218157

  • 744.00 SEK / 25 tests

In stock, ready to ship: 4

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Alert:

Contains Azide


Description

Description

Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs. Applications Reported: Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody. Applications Tested: Purified human Fc gammaR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μL per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
Specifications

Specifications

Fc Receptor Binding Inhibitor
Flow Cytometric Analysis
1 mg/mL
Unconjugated
25 Tests
Fc blocking; FcR Inhibitor; Fc Receptor Inhibitor; FcR BI; Fc Block; FcR Block; Fc receptor block; Gamma receptor block; Trustain; FcX; Fc blocking reagent; FcR blocking reagent
Affinity chromatography
Antibody
Polyclonal
Human
Flow Cytometry
Polyclonal
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Purified
Liquid
2° to 8°C
RUO
Primary
Human
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For Research Use Only