Globo H, clone: VK9, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9700-82
Additional Details : Weight : 0.09500kg
DescriptionThe VK9 antibody has also been tested by western blot of reduced MCF-7 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Globo H is a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.
|PBS with proprietary stabilizer and 0.09% sodium azide; pH 7.2|
|globoH; Globohexaosylceramide; Globo H antigen|
|Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot|
For Research Use Only.