Invitrogen™ IL-17AF (heterodimer) Human Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of human IL-17AF protein levels from serum, plasma and cell culture supernatants. This assay demonstrates parallelism in measuring recombinant and native human IL-17AF proteins and a standard curve range of 30 to 4000pg/mL.
Brand: Invitrogen™ 88-7117-22
Additional Details : Weight : 0.23750kg
DescriptionInterleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.This recombinant protein is E. coli expressed. Insect cell expressed Human IL-17A Recombinant Protein (cat. 14-8180) is also available.
|2°C to 8°C|
|24 hr. 30 min.|
|2 x 96 Tests|
|Colorimetric Microplate Reader|
|Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Assay Diluent: 5X concentrated, Wash Buffer: 10X concentrated (100 mL of 10X per plate), Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Stop Solution: 5 mLs of 1X solution per plate, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards|
|Plasma, 100 μL|Serum, 100 μL|Supernatant, 100 μL|
|1 hr. 45 min.|
For Research Use Only