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Invitrogen™ IL-17AF (heterodimer) Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of human IL-17AF protein levels from serum, plasma and cell culture supernatants. This assay demonstrates parallelism in measuring recombinant and native human IL-17AF proteins and a standard curve range of 30 to 4000pg/mL.

Brand:  Invitrogen™ 88-7117-86

3486.84 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.47500kg

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Product Code. 15581047

  • 5230.00 SEK / Pack of 10
Estimated Shipment: 08-05-2024
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Alert:

Contains Azide


Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Assay Diluent. 5X concentrated
Wash Buffer. 10X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Stop Solution

Description

Description

The Human IL-17AF (heterodimer) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17AF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.This recombinant protein is E. coli expressed. Insect cell expressed Human IL-17A Recombinant Protein (cat. 14-8180) is also available.
Specifications

Specifications

Q16552,Q96PD4
30 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
IL-5
HRP
RUO
2°C to 8°C
Human
24 hr. 30 min.
30-4,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
112744, 3605
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Assay Diluent: 5X concentrated, Wash Buffer: 10X concentrated (100 mL of 10X per plate), Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Stop Solution: 5 mLs of 1X solution per plate, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
10 x 96 Tests
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Interleukin-IL-17AF, IL17AF
1 hr. 45 min.
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