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Invitrogen™ IL1RA Human ELISA Kit

For the quantitative detection of human IL-1RA.

Brand:  Invitrogen™ BMS2080

4046.86 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.23750kg

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Product Code. 15561597

  • 6070.00 SEK / 96 tests
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal polyclonal antibody to human IL-1RA
Biotin-Conjugate anti-human IL-1RA polyclonal antibody
Streptavidin-HRP
Human IL-1RA Standard lyophilized, 14ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human Interleukin-1 Receptor Antagonist (Hu IL-1RA) ELISA quantitates Hu IL-1RA in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1RA. Principle of the method The Human IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 1 receptor antagonist (ILRA) is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13-14. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1alpha nor IL-1beta possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis.
Specifications

Specifications

P18510
30 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
DIRA,ICIL-1RA,IL-1RN,IL-1ra,IL-1ra3,IL1F3,IL1RA,IRAP,MVCD4
7.2%
HRP
RUO
2°C to 8°C
Human
3 hr. 30 min.
109-7,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3557
7.4%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Interleukin-1 receptor antagonist
1 hr. 20 min.
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