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  5. Invitrogen™ Human IL-2 ELISA Kit

Invitrogen™ Human IL-2 ELISA Kit

Produktkod. 15511727
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Kvantitet:
2 x 96 tester
5 x 96 tester
Förpackningsstorlek:
Paket med 2
Paket med 5
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Produktkod. 15511727

Brand: Invitrogen™ BMS2212FIVE

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Beskrivning

ELISA

The Human Interleukin-2 (Hu IL-2) ELISA quantitates Hu IL-2 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2. Principle of the method The Human IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
TRUSTED_SUSTAINABILITY

Specifications

Tillträdesnummer P60568
Analysintervall 18,8 till 1 200 pg/ml
Analyskänslighet 9.1 pg/mL
Konjugera HRP
Immunoassay Kit Format Sandwich ELISA Kit
Produkttyp ELISA
Provtyp Plasma, Serum, Supernatant
Målarter Människa
För användning med (applikation) ELISA
För användning med (utrustning) Kolorimetrisk mikroplattläsare
Gene Alias aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGFaldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF; IL-2; TCGF; lymphokine
Gen-ID (Entrez) 3558
Gensymbol IL2
Interassay CV 5%
Intraassay CV 7%
Kit Innehåll Förbelagd 96 brunnars platta, Standard, Provspädningsmedel, Analysbuffertkoncentrat, Biotinylerad detektionsantikropp, SAV-HRP, Tvättbuffert, Kontroller, Kromogen, Stoppa lösning, Självhäftande plåtskydd
Etikett eller färgämne Biotin
Proteinaliaser IL-2, T-cell growth factor, TCGF
Kvantitet 5 x 96 tester
Regulatorisk status RUO
Provvolym Plasma 50μ L, Serum 50μ L, Supernatant 50μ L
Förvaringskrav 2 °C till 8 °C
Synonym Aldesleukin, T-cellstillväxtfaktor, TCGF
Mål IL-2
Testtid 1 tim 20 min
Total analystid 3 timmar 30 minuter
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