Invitrogen™ IL-2 Human ELISA Kit, High Sensitivity

Innehåller

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-2
• Biotin-Conjugate anti-human IL-2 polyclonal antibody
• Streptavidin-HRP
• Human IL-2 Standard lyophilized, 2400pg/mL upon reconstitution
• Control high, lyophilized
• Control low, lyophilized
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Amplification Diluent Concentrate (2x)
• Amplification Reagent I
• Amplification Reagent II
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution
• Stop Solution (1M Phosphoric acid)
• Adhesive Films

High Sensitivity Sandwich ELISA Kit

The Human High Sensitivity Interleukin 2 (HS IL-2) ELISA quantitates Hu HS IL-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-2. Principle of the method The Human HS IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.