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Human IL-21, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8219-62

Additional Details : Weight : 0.09500kg

Product Code. 15517516

  • 1370.00 SEK / 5µg


Estimated Shipment date
from Supplier 27-06-2018
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Description

Description

IL-21 is a 17kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling innaive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. Description: Human Interleukin-21 (IL-21) is a 131-amino acid protein with 57% identity to the mouse gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma. Applications Reported: Recombinant Human IL-21 has been reported for use in cytokine bioassays, and ELISA. For research use only, not for diagnostic or therapeutic use. The recombinant human IL-21 has been reported useful for bioassay and ELISA. Applications Tested: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 25ng/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e4 Units/mg. Source: A DNA sequence encoding the mature form of human IL-21 amino acids Gln25-Ser155 accession # NM_021803 was expressed in E.coli. Bioactivity: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 25ng/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e4 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein has a predicted molecular mass of 17,150. The DTT reduced protein migrates as a 18kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 17kDa polypeptide. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Q9HBE4
Cytokine Bioassays , ELISA Recombinant Standard
PBS with 1% BSA and no preservative; pH 7.2
The protein has a predicted molecular mass of 17,150. The DTT reduced protein migrates as a 18kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 17kDa polypeptide.
7.2
Affinity chromatography
E. coli
RUO
Recombinant
Human IL-21
Control, ELISA, Functional Assay
Recombinant Protein
Unlabeled
E. coli
0.1mg/mL
Liquid
59067
Human IL-21
SDS-PAGE
5μg
-80°C
A DNA sequence encoding the mature form of human IL-21amino acids Gln25-Ser155 accession # NM_021803 was expressed in E.coli
Less than 0.01ng/ug cytokine as determined by the LAL assay.
IL21
The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 25ng/mL. This corresponds to a specific activity of greater than or equal to 4 x 104 Units/mg.
Human
Hu
Recombinant
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