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Invitrogen™ IL-31 Human ELISA Kit
For the quantitative detection of human IL-31.
Brand: Invitrogen™ BMS2041TEN
Additional Details : Weight : 0.23750kg
Includes: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-31
Biotin-Conjugate anti-human IL-31 polyclonal antibody
Streptavidin-HRP
Human IL-31 Standard lyophilized, 500pg/mL upon reconstitution
Sample Diluent
Conjugate Diluent
Calibrator Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Description
The Human Interleukin-31 (Hu IL-31) ELISA quantitates Hu IL-31 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-31. Principle of the method The Human IL-31 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 31, or IL-31 is a cytokine secreted by differentiated CD4+ T helper type 2 (Th2) cells. It is also released during inflammation of the skin, by macrophages and T cells. Because IL-31 is secreted by activated Th2 cells, it has a role in host defense against infection and may be involved in asthma, allergy and different types of bowel diseases.Specifications
Q6EBC2 | |
1.0 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
IL-31 | |
3.6% | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
4 hr. 30 min. |
7.8-500 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
386653 | |
2.9% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
10 x 96 Tests | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
Interleukin-31 | |
1 hr. 20 min. |
For Research Use Only