Invitrogen™ IL-6 Human Uncoated ELISA Kit

These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-6 (IL-6)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 10.2 to 400pg/mL

• Sensitivity: < 1pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).