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Invitrogen™ IL-7 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHIL7

5747.50 SEK valid until 2024-03-29
Use promo code "21653" to get your promotional price.



Additional Details : Weight : 0.00400kg

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Product Code. 15405053

  • 6050.00 SEK / 1 set
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Description

Description

The Human Interleukin 7 (Hu IL-7) ELISA quantitates Hu IL-7 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-7. Principle of the method The Human IL-7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
Specifications

Specifications

P13232
5 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
3574
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Immunology
2°C to 8°C
Human
4 hr. 45 min.
4.1-1000 pg/mL
5pg/mL to 1000pg/mL
Biotin
Other Proteins
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Human
Colorimetric Microplate Reader
IL-7
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
IL-7
1 hr. 20 min.
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